The chemical composition of bovine vitreous-humour collagen fibres

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The chemical composition of bovine vitreous-humour collagen fibres.

The insoluble protein fraction was prepared from the central and posterior peripheral fraction of bovine vitreous humour. The collagen present in this fraction was solubilized by pepsin and fractionated by gel chromatography. Analysis of the solubilized collagen fractions showed that the alpha-chain component had an amino acid composition and yielded a series of CNBr-cleavage peptides that show...

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A new look at vitreous-humour collagen.

The collagens of bovine vitreous-humour and nasal-septum cartilage have been extracted, fractionated and compared. Both tissues show the same heterogeneity of collagen types, consisting of type II, 1 alpha, 2 alpha, 3 alpha and C-PS collagens. The type II collagen of the vitreous humour was significantly more hydroxylated both in the lysine and proline residues than was that of cartilage. C-PS1...

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Viscosity and composition of solutions derived from rabbit vitreous humour.

THERE are three kinds of macromolecular substance in the vitreous humour: fibrous material, a viscous mucopolysaccharide, and soluble protein. The first, the residual protein, has many properties in common with collagen (Pirie, Schmidt, and Waters, 1948). The second, hyaluronic acid, has been characterized by Meyer and Palmer (1934) and shown to be a polymer of glucuronic acid and N-acetyl gluc...

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Rheological behaviour of vitreous humour

The vitreous humour (VH) is a complex biofluid that occupies a large portion of the eyeball between the lens and the retina, and exhibits non-Newtonian rheological properties that are key for its function in the eye. It is often possible to distinguish two different phases in VH, known as liquid and gel phases (Sebag J Eye 1: 254–262, 1987). In this work, we present a detailed rheological chara...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1980

ISSN: 0264-6021

DOI: 10.1042/bj1850545